Association between High Interferon-Gamma Production in Avian Tuberculin-Stimulated Blood from Mycobacterium avium subsp. paratuberculosis-Infected Cattle and Candidate Genes Implicated in Necroptosis

The mechanisms underlying host resistance to Mycobacterium avium subsp. paratuberculosis (MAP) infection are largely unknown. In the current study, we hypothesize that cows with an ability produce higher levels of interferon-gamma (IFNɣ) might control MAP more successfully. To test this hypothesis, IFNɣ production was measured using a specific ELISA kit in avian purified protein derivative (aPPD)-stimulated blood samples collected from 152 Holstein cattle. DNA isolated peripheral animals included study genotyped EuroG Medium-Density Bead Chip, and genotypes were imputed whole-genome sequencing. A genome-wide association analysis (GWAS) revealed high response aPPD associated genetic profile (heritability = 0.64) allowed identification 71 SNPs, 40 quantitative trait loci (QTL), 104 candidate genes. functional genes significant enrichment involved innate immune and, specifically, necroptosis. Taken together, our results define heritable distinct immunogenetic capacity lyse MAP-infected macrophages by